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Labcorp Oklahoma, Inc.
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ACTH (Adrenocorticotropic Hormone) Stimulation

Order Name ACTH STIM
Test Number: 2002151
Revision Date 10/01/2022
Test Name Methodology LOINC Code
Cortisol Baseline
Chemiluminescent Micropartical Immunoassay 43215-3 
Cortisol 30 Minute Specimen
Chemiluminescent Micropartical Immunoassay 12557-5 
Cortisol 60 Minute Specimen
Chemiluminescent Micropartical Immunoassay 12558-3 
SPECIMEN REQUIREMENTS
Specimen Specimen Volume (min) Specimen Type Specimen Container Transport Environment
Preferred 1 mL (0.5) Serum Clot Activator SST Refrigerated or Frozen
Alternate 1 1 mL (0.5) Plasma Lithium Heparin PST (Light Green Top) Refrigerated or Frozen
Instructions This test needs to be scheduled for Collection. See information in the Notes Section below.
Stability: Ambient 1 day. Refrigerated 14 days. Frozen 30 days.
GENERAL INFORMATION
Testing Schedule Mon - Fri 
Expected TAT 1-3 days  
Clinical Use This test determines how the adrenal glands respond to a certain hormone. The hormone is called adrenocorticotrophic hormone, also known as ACTH. ACTH is made in the pituitary gland and travels through the bloodstream to the adrenal glands. ACTH stimulates the adrenal gland to release Cortisol. Cortisol is a key factor in many functions in the body's metabolism of fats, carbohydrates, sodium, potassium, and protein. 
Notes
Testing Locations
Tulsa

Cortisol (ACTH) Stimulations may be scheduled with Labcorp Oklahoma, Inc. Patient Service Center at St. John Medical Center for collections M-F 7:30am to 4:00pm at (918) 744-3131 x 15531; after 4:00pm may be scheduled with Chemistry department at (918)744-3131 x 15514. Testing is performed Monday through Friday 9:00am to 11am. and requires about 1 hour and 15 minutes of the patient's time.
Bartlesville

Cortisol (ACTH) stimulations may be scheduled with Labcorp Oklahoma, Inc. at Jane Phillips Medical Center, Monday through Friday at (918) 331-1737.
Physician Office

If the testing is performed in the physician's office, the following protocol is suggested.
ACTH Stimulation Test
  1. Baseline Cortisol - Have a Cortisol drawn prior to injection (5 ml gold top tube). Note the time drawn on the tube.
  2. Administer 250 micrograms (1 vial) of synthetic ACTH Cortrosyn (Cosyntropin) intravenously. Use supplied diluents to dissolve ACTH powder. Inject over 1-2 minutes.
  3. 30 minute Cortisol - draw one clot tube for a Cortisol level 30 minutes after the injection. Note the time drawn on the tube.
  4. 60 minute Cortisol - draw a second clot tube for a Cortisol level 60 minutes after the injection. Note the time drawn on the tube.
CPT Code(s) 80400, 82533, 80503
Lab Section Chemistry