Bordetella pertussis/parapertussis DNA, Qualitative Real-Time PCR
Test Number: 5568100
Revision Date 10/01/2022
| Test Name | Methodology | LOINC Code |
|---|---|---|
|
Bordetella pertussis DNA
|
Polymerase Chain Reaction | 23826-1 |
|
Bordetella parapertussis DNA
|
Polymerase Chain Reaction | 29723-4 |
| SPECIMEN REQUIREMENTS | ||||
|---|---|---|---|---|
| Specimen | Specimen Volume (min) | Specimen Type | Specimen Container | Transport Environment |
| Preferred | See Instructions | Swab | Flocked Flexible Mini-Tip Nasopharyngeal Swab | Refrigerated |
| Alternate 1 | See Instructions | Nasal Wash | Sterile Screwtop Container | Refrigerated |
| Instructions | USE ONE OF TWO COLLECTION METHODS:
1) Universal Transport Media (UTM) with mini-Flocked Swab (Comes as a kit: Supply# 50775). Collect a nasopharyngeal specimen leaving the swab in place for a few seconds to absorb secretions. Swab both nostrils and place swab immediately into a single sterile common UTM container. - KEEP REFRIGERATED (Alternate Swab: AMIES Blue Cap Swab in UTM - Refrigerated.) 2) Nasopharyngeal Aspirates (Collect in the Physician's office): Flush each nostril with 1mL to 1.5ml of Nonbacteriostatic Saline (pH 7.0) - Collect the drainage from each nostril into a common sterile container. - KEEP REFRIGERATED Caution: DO NOT use Calcium Alginate Swabs or ESwabs as they will inhibit PCR testing. DO NOT put Swabs in Charcoal Transport Media. Specimen Stability: |
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| GENERAL INFORMATION | |
|---|---|
| Testing Schedule | Mon, Wed, Fri |
| Expected TAT | 2-3 days |
| Notes | Bordetella pertussis is the cause of whooping cough that may occur in unimmunized individuals. B. parapertussis is a related organism that causes a similar but milder disease. Laboratory diagnosis may require both culture and serological confirmation although culture is difficult. |
| CPT Code(s) | 87798x2 |
| Lab Section | Molecular Diagnostic |