| GEL | MTS Gel Technology, Ortho Clinical Diagnostics | (GEL) Red blood cells that are coated with IgG due to in vitro sensitization are determined indirectly by the MTS Anti-IgG Gel cards. The MTS gel card restricts the unbound IgG from moving through the gel during centrifugation. Thus, unbound IgG does not neutralize the Anti-IgG incorporated in the gel. IgG coated red cells will react with the Anti-IgG gel in the microtube during centrifugation. Strong positive reaction will produce a red line of cells layered at the top of the gel. Weak positive reactions will have visible red cell agglutinates suspended throughout the gel. Uncoated (non sensitized) cells or cells coated only with complement are not agglutinated by the Anti-IgG gel and will form a button at the bottom of the microtube. |