EP | Electrophoresis | (EP) is a technique for separation of ionic molecules (principally proteins) by the differential migration through a gel according to the size and ionic charge of the molecules in an electrical field. Smaller molecules with a more negative charge will travel faster and further through the gel toward the anode of an electrophoretic cell when high voltage is applied. Similar molecules will group on the gel. They may be visualized by staining and quantitated, in relative terms, using densitometers which continuously monitor the photometric density of the resulting stain. |